{"id":7997,"date":"2020-11-17T13:39:16","date_gmt":"2020-11-17T13:39:16","guid":{"rendered":"https:\/\/cdtrp.ca\/?p=7997"},"modified":"2020-11-17T13:39:16","modified_gmt":"2020-11-17T13:39:16","slug":"journal-club-de-last-ajt-24-novembre-dre-megan-levings","status":"publish","type":"post","link":"https:\/\/cdtrp.ca\/fr\/journal-club-de-last-ajt-24-novembre-dre-megan-levings\/","title":{"rendered":"Journal Club de l&rsquo;AST\/AJT &#8211; 24 novembre : Dre Megan Levings"},"content":{"rendered":"<div class=\"fusion-fullwidth fullwidth-box fusion-builder-row-1 fusion-flex-container nonhundred-percent-fullwidth non-hundred-percent-height-scrolling\" style=\"--awb-border-radius-top-left:0px;--awb-border-radius-top-right:0px;--awb-border-radius-bottom-right:0px;--awb-border-radius-bottom-left:0px;--awb-flex-wrap:wrap;\" ><div class=\"fusion-builder-row fusion-row fusion-flex-align-items-flex-start fusion-flex-content-wrap\" style=\"max-width:1216.8px;margin-left: calc(-4% \/ 2 );margin-right: calc(-4% \/ 2 );\"><div class=\"fusion-layout-column fusion_builder_column fusion-builder-column-0 fusion_builder_column_1_1 1_1 fusion-flex-column\" style=\"--awb-bg-size:cover;--awb-width-large:100%;--awb-margin-top-large:0px;--awb-spacing-right-large:1.92%;--awb-margin-bottom-large:0px;--awb-spacing-left-large:1.92%;--awb-width-medium:100%;--awb-order-medium:0;--awb-spacing-right-medium:1.92%;--awb-spacing-left-medium:1.92%;--awb-width-small:100%;--awb-order-small:0;--awb-spacing-right-small:1.92%;--awb-spacing-left-small:1.92%;\"><div class=\"fusion-column-wrapper fusion-column-has-shadow fusion-flex-justify-content-flex-start fusion-content-layout-column\"><div class=\"fusion-text fusion-text-1\"><h3><em>Journal Club<\/em> de l&rsquo;AST\/AJT &#8211; 24 novembre : Dre Megan Levings<\/h3>\n<p>L&rsquo;<a href=\"https:\/\/www.myast.org\/\">American Society of Transplantation<\/a> (AST) et l&rsquo;<a href=\"https:\/\/onlinelibrary.wiley.com\/journal\/16006143\">American Journal of Transplantation<\/a> (AJT) invitent la communaut\u00e9 de transplantation \u00e0 rejoindre le <em>Journal Club<\/em> bas\u00e9 sur cet article publi\u00e9 dans <em>Science Translational Medicine<\/em> en ao\u00fbt 2020 :\u00a0<a href=\"https:\/\/stm.sciencemag.org\/content\/12\/557\/eaaz3866\" target=\"_blank\" rel=\"noopener noreferrer\">\u00ab\u00a0Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells.\u00a0\u00bb<\/a> (en anglais)<\/p>\n<h5 data-pm-slice=\"1 1 &#091;&#093;\">24 novembre 2020 \u00e0 14h00 HE &#8211; <a href=\"https:\/\/register.gotowebinar.com\/register\/7068163630296060944\">Inscrivez-vous ici.<\/a><\/h5>\n<h5 data-pm-slice=\"1 1 &#091;&#093;\">Conf\u00e9renci\u00e8re :<\/h5>\n<ul>\n<li>Megan Levings, PhD \u2022 <em>University of British Columbia<\/em>, Vancouver, BC<\/li>\n<\/ul>\n<h5>Mod\u00e9ratrice :<\/h5>\n<ul>\n<li>Olivia Martinez, PhD \u2022 <em>Stanford University<\/em>, Stanford, CA<\/li>\n<\/ul>\n<h5>Invit\u00e9s Questions-R\u00e9ponses :<\/h5>\n<ul>\n<li>Nicholas Dawson, PhD \u2022 <em>University of British Columbia<\/em>, Vancouver, BC<\/li>\n<li>Isaac Rosado-S\u00e1nchez, PhD \u2022 <em>BC Children&rsquo;s Hospital Research Institute<\/em>, Vancouver, BC<\/li>\n<\/ul>\n<p><i>Les <\/i>Journal clubs<i> sont gratuits pour les membres et les non-membres. Une pr\u00e9-inscription est n\u00e9cessaire.<\/i><\/p>\n<\/div><div class=\"fusion-separator fusion-full-width-sep\" style=\"align-self: center;margin-left: auto;margin-right: auto;margin-top:20px;margin-bottom:20px;width:100%;\"><\/div><div class=\"fusion-text fusion-text-2\"><h4>R\u00e9sum\u00e9 de l&rsquo;article (en anglais)<\/h4>\n<p><em>Antigen-specific regulatory T cells (T<sub>regs<\/sub>) engineered with chimeric antigen receptors (CARs) are a potent immunosuppressive cellular therapy in multiple disease models and could overcome shortcomings of polyclonal T<sub>reg<\/sub>\u00a0therapy. CAR therapy was initially developed with conventional T cells, which have different signaling requirements than do T<sub>regs<\/sub>. To date, most of the CAR T<sub>reg<\/sub>\u00a0studies used second-generation CARs, encoding a CD28 or 4-1BB co-receptor signaling domain and CD3\u03b6, but it was not known if this CAR design was optimal for T<sub>regs<\/sub>. Using a human leukocyte antigen\u2013A2\u2013specific CAR platform and human T<sub>regs<\/sub>, we compared 10 CARs with different co-receptor signaling domains and systematically tested their function and CAR-stimulated gene expression profile. T<sub>regs<\/sub>\u00a0expressing a CAR encoding CD28wt were markedly superior to all other CARs tested in an in vivo model of graft-versus-host disease. In vitro assays revealed stable expression of Helios and an ability to suppress CD80 expression on dendritic cells as key in vitro predictors of in vivo function. This comprehensive study of CAR signaling domain variants in T<sub>regs<\/sub>\u00a0can be leveraged to optimize CAR design for use in antigen-specific T<sub>reg<\/sub>\u00a0therapy.<\/em><\/p>\n<h4 style=\"text-align: center;\"><a href=\"https:\/\/register.gotowebinar.com\/register\/7068163630296060944\">Cliquez ici pour vous inscrire (gratuit)<\/a><\/h4>\n<\/div><\/div><\/div><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":6,"featured_media":7999,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[81,37],"tags":[],"class_list":["post-7997","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-en-vedette","category-non-classifiee"],"_links":{"self":[{"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/posts\/7997"}],"collection":[{"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/comments?post=7997"}],"version-history":[{"count":3,"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/posts\/7997\/revisions"}],"predecessor-version":[{"id":8070,"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/posts\/7997\/revisions\/8070"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/media\/7999"}],"wp:attachment":[{"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/media?parent=7997"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/categories?post=7997"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cdtrp.ca\/fr\/wp-json\/wp\/v2\/tags?post=7997"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}