{"id":6485,"date":"2020-08-24T18:08:36","date_gmt":"2020-08-24T18:08:36","guid":{"rendered":"https:\/\/cdtrp.ca\/?p=6485"},"modified":"2020-08-24T18:08:36","modified_gmt":"2020-08-24T18:08:36","slug":"article-en-vedette-functional-effects-of-chimeric-antigen-receptor-co-receptor-signaling-domains-in-human-regulatory-t-cells","status":"publish","type":"post","link":"https:\/\/cdtrp.ca\/fr\/article-en-vedette-functional-effects-of-chimeric-antigen-receptor-co-receptor-signaling-domains-in-human-regulatory-t-cells\/","title":{"rendered":"Article en vedette : Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells"},"content":{"rendered":"

Article en vedette : Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells<\/em><\/h4>\n

Le PRDTC tient \u00e0 f\u00e9liciter Megan Levings et tous ses collaborateurs qui ont travaill\u00e9 sur l’article \u00ab\u00a0Functional effects of chimeric antigen receptor co-receptor signaling domains in human regulatory T cells<\/strong><\/em>\u00ab\u00a0, r\u00e9cemment publi\u00e9 dans Science – Translational Medicine<\/em>!<\/span><\/p>\n

R\u00e9sum\u00e9 (en anglais)<\/h5>\n

Antigen-specific regulatory T cells (Tregs) engineered with chimeric antigen receptors (CARs) are a potent immunosuppressive cellular therapy in multiple disease models and could overcome shortcomings of polyclonal Treg therapy. CAR therapy was initially developed with conventional T cells, which have different signaling requirements than do Tregs. To date, most of the CAR Treg studies used second-generation CARs, encoding a CD28 or 4-1BB co-receptor signaling domain and CD3\u03b6, but it was not known if this CAR design was optimal for Tregs. Using a human leukocyte antigen\u2013A2\u2013specific CAR platform and human Tregs, we compared 10 CARs with different co-receptor signaling domains and systematically tested their function and CAR-stimulated gene expression profile. Tregs expressing a CAR encoding CD28wt were markedly superior to all other CARs tested in an in vivo model of graft-versus-host disease. In vitro assays revealed stable expression of Helios and an ability to suppress CD80 expression on dendritic cells as key in vitro predictors of in vivo function. This comprehensive study of CAR signaling domain variants in Tregs can be leveraged to optimize CAR design for use in antigen-specific Treg therapy.<\/p>\n

Vous pouvez lire l’article ici<\/a> (en anglais).<\/h5>\n<\/div>
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