{"id":20280,"date":"2022-10-26T13:26:33","date_gmt":"2022-10-26T18:26:33","guid":{"rendered":"https:\/\/cdtrp.ca\/en\/?post_type=spucpt&p=20280"},"modified":"2022-11-02T10:52:18","modified_gmt":"2022-11-02T15:52:18","slug":"marwa-sadat-3mt","status":"publish","type":"spucpt","link":"https:\/\/cdtrp.ca\/en\/spucpt\/marwa-sadat-3mt\/","title":{"rendered":"Marwa Sadat 3MT"},"content":{"rendered":"

Marwa Sadat’s bio<\/strong><\/p>\n

\"\"<\/p>\n

Marwa is a 1st year Masters student in Dr. Ian Rogers lab at the University of\u00a0Toronto in the Department of Physiology. She completed her Bachelor of Science in Life Sciences at the University of Toronto, with a double major in Physiology and Neuroscience. In her research, she is interested in using ex vivo perfusion of the mouse and humanized pancreas to study type 1 diabetes. On one hand, she is working on optimizing ex vivo murine pancreas perfusion and using it to model type 1 diabetes. Simultaneously, she is focused on generating a humanized pancreas through decellularization-recellularization of the mouse pancreas with human progenitor cells, based on the idea that the extracellular matrix will guide maturation of progenitors. During her free time, she enjoys discovering new hiking trails and trying new restaurants around the city.<\/p>\n

\n

Normothermic ex vivo perfusion of the murine pancreas to model type 1 diabetes<\/strong><\/p>\n

Marwa Sadat, Jorge Castillo-Prado, Ian M. Rogers<\/em><\/p>\n

Diseases of the pancreas affect hundreds of millions worldwide, including diabetes, pancreatitis, and pancreatic cancer, for which current treatment methods are insufficient, and which lack proper disease modelling. Current animal models for pancreatic disease are useful, however they can be complicated, invasive, and can result in immediate fatality following disease generation. Our goal is to generate a normothermic ex vivo organ perfusion (EVOP) system for the pancreas that mimics the in vivo environment, where the pancreas can be monitored and assayed in real-time, which is limited in animal models. Previously, we have mathematically designed and built an EVOP system that can successfully support the whole adult murine kidney for upto 9 days, by providing it with sufficient oxygen, nutrients, and hydrostatic support in a sterile manner. In our current study, preliminary data shows that our EVOP system can support the native murine pancreas for up to 4 days, as indicated by H&E staining which reveals healthy-looking tissue, as well as a positive glucose-stimulated insulin secretion (GSIS) test indicating healthy endocrine function. Currently, culture conditions for longer term experiments are being optimized, by adding various nutrients and supplements to best support the metabolic needs of the pancreas. Next, we will generate a model of type 1 diabetes mellitus (T1DM) in the isolated pancreas by perfusing the organ with the highly selective pancreatic \u03b2 cell cytotoxin streptozotocin (STZ), which is effective in generating T1DM in vivo in rodents. Generation of T1DM will be confirmed via disappearance of the insulin spike in the GSIS test post-STZ perfusion. This system will not only allow for successful generation of ex vivo models of pancreatic disease which can be used to test various therapeutic agents in an isolated manner, but can also aid in furthering efforts for maintaining human organs for transplants.<\/p>\n","protected":false},"author":6,"template":"","class_list":["post-20280","spucpt","type-spucpt","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/spucpt\/20280"}],"collection":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/spucpt"}],"about":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/types\/spucpt"}],"author":[{"embeddable":true,"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/users\/6"}],"wp:attachment":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/media?parent=20280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}