{"id":14189,"date":"2021-11-10T13:50:28","date_gmt":"2021-11-10T18:50:28","guid":{"rendered":"https:\/\/cdtrp.ca\/en\/?post_type=spucpt&#038;p=14189"},"modified":"2021-11-10T13:50:28","modified_gmt":"2021-11-10T18:50:28","slug":"jonathan-choy-talk","status":"publish","type":"spucpt","link":"https:\/\/cdtrp.ca\/en\/spucpt\/jonathan-choy-talk\/","title":{"rendered":"Jonathan Choy talk"},"content":{"rendered":"<h4><strong>Cell surface engineering of the endothelial\u00a0glycocalyx\u00a0for prevention of transplant rejection\u00a0<\/strong><\/h4>\n<p>The endothelial\u00a0glyxocalyx\u00a0is a glycoprotein-rich coating on the luminal surface of vascular\u00a0endothelial cells that has important immune modulatory properties.\u00a0 It is particularly susceptible to injury during organ procurement\u00a0and this may play a role in triggering early graft inflammation and rejection.\u00a0\u00a0Rebuilding the endothelial\u00a0glycocalyx by enzymatic\u00a0 conjugation of linear\u00a0polyglycerol\u00a0polymers containing the bioactive sugar\u00a0sialic\u00a0acid onto the surface of human endothelial cells reduced leukocyte binding and inhibited leukocyte-mediated cytotoxicity of endothelial cells.\u00a0 Application of this technology\u00a0to artery grafts in a\u00a0mouse model led to substantial reductions in early inflammation, and acute and chronic rejection. Also, this\u00a0cell surface engineering\u00a0of vascular endothelial cells in mouse kidney transplant models reduced ischemia-mediated kidney graft failure\u00a0and\u00a0allograft rejection.\u00a0 This work\u00a0provides\u00a0a foundation for the development a novel cell surface engineering protocol that\u00a0can be applied to donor organs at the time of procurement and\u00a0protects\u00a0them\u00a0from rejection.<\/p>\n","protected":false},"author":6,"template":"","class_list":["post-14189","spucpt","type-spucpt","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/spucpt\/14189"}],"collection":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/spucpt"}],"about":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/types\/spucpt"}],"author":[{"embeddable":true,"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/users\/6"}],"wp:attachment":[{"href":"https:\/\/cdtrp.ca\/en\/wp-json\/wp\/v2\/media?parent=14189"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}